Titulação: Doutor
Laboratório:Vascular Nanopharmacology
Telefone: 55 (11) 3091-7232
E-mail: stephen.rodrigues@usp.br

Linha de Pesquisa

To study the effect of nanodrugs on the microcirculation in different diseases.

Descrição

Microcirculation (arterioles, venules, capillaries, lymphatic vessels) is of great importance to guide drugs to their targets, is itself a site of action of several drugs, and is fundamental for the drugs to leave the body. Abnormal changes on the microcirculation (enhanced leukocyte and platelet adhesion and migration, unbalanced thrombus formation, excess of reactive oxygen and nitrogen species formation, enhanced vascular permeability, among others) occur in several diseases that affect great part of the population in the world, such as, hypertension, stroke, cancer, inflammatory disease (multiple sclerosis, sepsis, acute respiratory distress syndrome). This way, a continuing search for new drugs or drugs repositioning with focus on reverting or preventing microcirculation changes and then improving disease states is our goal. Lastly, we have turned our attention to nanodrugs (drugs with at least one of their dimensions at the nanoscale) that act on the microcirculation in different tissues, including the central nervous system (CNS), lung, testis, and mesentery, aiming to prevent or treat diseases. To do that, our lab has used mainly the following experimental assays: fluorescence intravital microscopy, flow cytometry,immunohistochemistry, western blotting, ELISA, Evans blue permeability assay, Boyden chamber-based cell migration.

Principais Trabalhos Desenvolvidos

• 1. DI BELLA D, FERREIRA JPS, SILVA RNO, ECHEM C, MILAN A, AKAMINE EH, CARVALHO MH, RODRIGUES, SF. Gold nanoparticles reduce inflammation in cerebral microvessels of mice with sepsis. Journal of Nanobiotechnology, v. 19, p. 52, 2021.
• 2. SILVA VCJ, SILVA RNO, COLLI LG, CARVALHO MHC, RODRIGUES, SF. Gold nanoparticles carrying or not anti-VEGF antibody do not change glioblastoma multiforme tumor progression in mice. Heliyon, v. 6, p. e05591, 2020.
• 3. COLLI LG, BELARDIN LB, ECHEM C, AKAMINE EH, ANTONIASSI MP, ANDRETTA RR, MATHIAS LS, RODRIGUES SF, BERTOLLA RP, CARVALHO MHC. Systemic arterial hypertension leads to decreased semen quality and alterations in the testicular microcirculation in rats. Scientific Reports, v. 9, p. 11047, 2019.
• 4. RODRIGUES SF, FIEL LA, SHIMADA AL, PEREIRA NR, GUTERRES SS, POHLMANN AR, FARSKY SH. Lipid-Core Nanocapsules Act as a Drug Shuttle Through the Blood Brain Barrier and Reduce Glioblastoma After Intravenous or Oral Administration. Journal of Biomedical Nanotechnology, v.12, p. 986-1000, 2016.
• 5. UCHIYAMA MK, DEDA DK, RODRIGUES SF, DREWES CC, BOLONHEIS SM, KIYOHARA PK, DE TOLEDO SP, COLLI W, ARAKI K, FARSKY SHP. In Vivo and In Vitro Toxicity and Anti-Inflammatory Properties of Gold Nanoparticle Bioconjugates to the Vascular System. Toxicological Sciences (Print), v. 142, p. 497-507, 2014.
• 6. RODRIGUES SF, VITAL SA, GRANGER DN. Mild hypercholesterolemia blunts the proinflammatory and prothrombotic effects of hypertension on the cerebral microcirculation. Journal of Cerebral Blood Flow & Metabolism, v. 33, p. 483-489, 2013.
• 7. RODRIGUES SF, Granger DN. Cerebral microvascular inflammation in DOCA salt-induced hypertension: role of angiotensin II and mitochondrial superoxide. Journal of Cerebral Blood Flow & Metabolism, v. 32, p. 368-375, 2011.
• 8. RODRIGUES SF, TRAN ED, FORTES ZB, SCHMID-SCHONBEIN GW. Matrix metalloproteinases cleave the 2-adrenergic receptor in spontaneously hypertensive rats. American Journal of Physiology. Heart and Circulatory Physiology, v. 299, p. H25-H35, 2010.Vídeo: Cerebral venule of hypertensive mouse fed a high cholesterol diet showing enhanced leukocyte (labeled in red) and platelet (labeled in green) rolling and adhesion

Video 3 Stephen R